investigating the association of il-17a and il-17f with susceptibility to pre-eclampsia in iranian women
نویسندگان
چکیده
background: pre-eclampsia (pe) is one of the most important and life-threatening pregnancy disorders that affect at least 3-5% of all pregnancies. imbalance in helper t cell functions may play a role in predisposing to pe or severity of the disease. elevated frequencies of th17 cells in the peripheral blood of pe patients have been reported. several single nucleotide polymorphisms (snp) within il-17 gene have been identified that may affect the il-17 production. objectives: to investigate the association between il-17a (-197a/g) and il-17f (+7488t/c) gene polymorphisms and susceptibility to pe in a group of iranian women. moreover, to study any correlation of the polymorphisms data with the level of il-17, at mrna level in the paternal and maternal parts of the placentas and also at protein level in the peripheral and placental blood samples. methods: a group of 261 pe patients and 278 age-matched healthy women with at least two previous normal pregnancies formed the cases and controls of this study. il-17a (-197a/g) and il-17f (+7488t/c) polymorphisms were genotyped using pcr-rflp method. the protein level of il-17a was assessed in the sera of 40 pe and 40 healthy women using elisa method and mrna expression was also measured in placental samples of 19 pe and 19 control women using q-pcr technique. results: statistical analysis indicated that there were no differences in genotype, allele or haplotype frequencies regarding the studied snps between cases and controls. the level of il-17a was elevated in the placental blood and the fetal tissue at protein and mrna levels (p< 0.009 and p
منابع مشابه
Investigating the Association of IL-17A and IL-17F with Susceptibility to Pre-eclampsia in Iranian Women
Background: Pre-eclampsia (PE) is one of the most important and life-threatening pregnancy disorders that affect at least 3-5% of all pregnancies. Imbalance in helper T cell functions may play a role in predisposing to PE or severity of the disease. Elevated frequencies of Th17 cells in the peripheral blood of PE patients have been reported. Several single nucleotide polymorphisms (SNP) within ...
متن کاملAssociation analysis of IL-17A and IL-17F polymorphisms in Chinese women with cervical cancer.
We selected six tagged single nucleotide polymorphisms (SNPs) in the interleukin 17A (IL-17A) and IL-17F genes, and evaluated the relationship between the six common SNPs and environmental factors in cervical cancer patients. Polymerase chain reaction-restriction fragment length polymorphism was used to detect the IL-17A (rs2275913, rs3748067, and rs3819025) and IL-17F (rs763780, rs9382084, and...
متن کاملAssociation Analysis of IL-17A and IL-17F Polymorphisms in Chinese Han Women with Breast Cancer
BACKGROUND Research into the etiology of breast cancer has recently focused on the role of the immunity and inflammation. The proinflammatory cytokines IL-17A and IL-17F can mediate inflammation and cancer. To evaluate the influences of IL-17A and IL-17F gene polymorphisms on the risk of sporadic breast cancer, a case-control study was conducted in Chinese Han women. METHODOLOGY AND PRINCIPAL...
متن کاملIdentification of cells expressing IL-17A and IL-17F in the lungs of patients with COPD.
BACKGROUND Lymphocytes secrete IL-17A and IL-17F, which enhance innate immune responses. IL-17 expression has not been studied in COPD small airways. The aim of this study was to quantify IL-17A and IL-17F expression in the peripheral lung tissue of patients with COPD compared with control subjects and to identify inflammatory cells that express IL-17. METHODS IL-17 expression was assessed us...
متن کاملInvestigating the contribution of IL-17A and IL-17F to the host response during Escherichia coli mastitis
Mastitis remains a major disease of cattle with a strong impact on the dairy industry. There is a growing interest in understanding how cell mediated immunity contributes to the defence of the mammary gland against invading mastitis causing bacteria. Cytokines belonging to the IL-17 family, and the cells that produce them, have been described as important modulators of the innate immunity, in p...
متن کاملGenetic polymorphisms of IL-17A, IL-17F, TLR4 and miR-146a in association with the risk of pulmonary tuberculosis
Genetic factors affect host susceptibility to pathogens. In this population-based case control study, we explored the genetic polymorphisms of IL-17, TLR4 and miR-146a in association with pulmonary tuberculosis in a Chinese Han population. We recruited 1601 pulmonary tuberculosis patients matched with 1526 healthy controls and genotyped twelve functional single nucleotide polymorphisms (SNPs). ...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of immunologyجلد ۱۲، شماره ۲، صفحات ۱۱۷-۱۲۸
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023